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Fig1: Western blot analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: HepG2
Fig2: ICC staining 14-3-3 alpha+beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining 14-3-3 alpha+beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining 14-3-3 alpha+beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of Hela cells with 14-3-3 alpha+beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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