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| 銷售商： 杭州華安生物技術有限公司 | 查看該公司所有產品 >> |
| 抗體類型 | 兔多抗 | 產品類型 | 一抗 |
| 反應種屬 | Zebrafish , Rat , Mouse , Human | 產品應用 | WB |
| 儲藏條件 | 產品性狀 | 液體 | |
| 分子量 | 36kDa | 克隆號 | |
| 保質期 | 12個月 | 產品品牌 | 華安 |
| 細胞定位 | 細胞質 | 陽性對照 | MCF-7 |
抗原介紹
應用介紹
【免疫原】免疫原為KLH-多肽交聯物，多肽位于C-末端附近區域
【推薦使用濃度】WB: 1:10,000
Fig1: Western blot analysis of Cyclin D1 on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining Cyclin D1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
Fig3: ICC staining Cyclin D1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
Fig4: ICC staining Cyclin D1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin D1 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. AlexaFluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
