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Fig1: Western blot analysis of EZR on human EZR recombinant protein using anti-EZR antibody at 1/1,000 dilution.
Fig2: Western blot analysis of EZR on HEK293 (1) and EZR-hIgGFc transfected HEK293 (2) cell lysate using anti-EZR antibody at 1/1,000 dilution.
Fig3: Western blot analysis of EZR on different cell lysate using anti-EZR antibody at 1/1,000 dilution. Positive control： Line1: MCF-7 Line2: Hela Line3: A431 Line4: Hek293 Line5: SK-N-SH Line6: Jurkat Line7: HepG2 Line8: NIH/3T3 Line9: Cos7
Fig4: ICC staining EZR (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of Hela cells with EZR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Fig6: Flow cytometric analysis of MCF-7 cells with EZR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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