Fig1: Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1,000 dilution.
Fig2: ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
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杭州華安生物技術有限公司成立于2007年3月，總部位于中國杭州，是一家從事抗體研發(fā)與生產(chǎn)的國家高新技術企業(yè)。公司致力于為全球的科研工作者和工業(yè)客戶提供高品質的抗體試劑及抗體服務。
公司的產(chǎn)品線包括兔單抗、小鼠單抗、兔多抗、二抗、蛋白/多肽、基因敲除細胞系、抗體檢測用相關試劑等。2015年，公司開始專注于高通量重組兔單克隆抗體的研發(fā)與產(chǎn)業(yè)化生產(chǎn)。
同時，公司也為客戶提供定制抗體的開發(fā)、純化和篩選服務。迄今為止，公司已累計完成超過 3000個定制抗體項目，涉及植物、動物、醫(yī)學、水產(chǎn)、病毒等不同的科研領域。用戶使用公司抗體在Science、Immunity、Nature Communications、Autophagy、Plant Cell、Cell Research等雜志上發(fā)表文章。
公司不但專注于自身技術體系的完善和升級，還注重推動產(chǎn)學研合作：與浙江大學、四川大學、西南大學、中國科學院遺傳與發(fā)育生物學研究所等知名院校共同承擔省級和國家級課題，與杭州精準醫(yī)藥研究中心進行深度合作，進行技術成果交流和共享。
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