Fig1: Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1,000 dilution.
Fig2: ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
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杭州華安生物技術有限公司成立于2007年3月，總部位于中國杭州，是一家從事抗體研發與生產的國家高新技術企業。公司致力于為全球的科研工作者和工業客戶提供高品質的抗體試劑及抗體服務。
公司的產品線包括兔單抗、小鼠單抗、兔多抗、二抗、蛋白/多肽、基因敲除細胞系、抗體檢測用相關試劑等。2015年，公司開始專注于高通量重組兔單克隆抗體的研發與產業化生產。
同時，公司也為客戶提供定制抗體的開發、純化和篩選服務。迄今為止，公司已累計完成超過 3000個定制抗體項目，涉及植物、動物、醫學、水產、病毒等不同的科研領域。用戶使用公司抗體在Science、Immunity、Nature Communications、Autophagy、Plant Cell、Cell Research等雜志上發表文章。
公司不但專注于自身技術體系的完善和升級，還注重推動產學研合作：與浙江大學、四川大學、西南大學、中國科學院遺傳與發育生物學研究所等知名院校共同承擔省級和國家級課題，與杭州精準醫藥研究中心進行深度合作，進行技術成果交流和共享。
Hangzhou HuaAn Biotechnology Co., Ltd (HuaBio) was founded in 2007. Our headquarter is located in Hangzhou, China. With over 3,000 antibodies developed, HuaBio is committed to providing high-quality antibodies and antibody services to our customers. We have products including recombinant rabbit monoclonal antibodies, mouse monoclonal antibodies, rabbit polyclonal antibodies, phosphor-specific antibodies, secondary antibodies.
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