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Fig1: Western blot analysis of TRIM28 on different cell lysates using anti-TRIM28 antibody at 1/1,000 dilution.
Fig2: ICC staining TRIM28 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRIM28 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of Hela cells with TRIM28 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
