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Fig1: Western blot analysis of PDK1 on rat heart tissue lysate using anti-PDK1 antibody at 1/1,000 dilution.
Fig2: ICC staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PDK1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDK1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PDK1 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of NIH-3T3 cells with PDK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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