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Fig1: Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution. Positive control: Lane 1: 293T Lane 2:MCF-7
Fig2: ICC staining CTCF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CTCF in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining CTCF in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Fig10: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CTCF antibody. Counter stained with hematoxylin.
Fig11: Flow cytometric analysis of 293T cells with CTCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Fig12: Western blot analysis of CTCF on Zebrafish tissue lysates using anti-CTCF antibody at 1/200 dilution.
Fig13: Western blot analysis of CTCF on hybrid fish (crucian-carp) brain tissue lysate using anti-CTCF antibody at 1/500 dilution.
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