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Fig1: Western blot analysis of eIF4EBP1 on different lysates using anti-eIF4EBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: 293
Fig2: ICC staining eIF4EBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining eIF4EBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of NIH/3T3 cells with eIF4EBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
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