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Fig1: Western blot analysis of Calreticulin on different lysates using anti-Calreticulin antibody at 1/1,000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: HL-60
Fig2: ICC staining Calreticulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Calreticulin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Calreticulin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: ICC staining Calreticulin in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of Hela cells with Calreticulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
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