Fig1: Western blot analysis of Phospho-Akt1(Ser473) on different lysates using anti-Phospho-Akt1(Ser473) antibody at 1/1,000 dilution. Positive control:
Lane 1: NIH/3T3 treated with PDGF
Lane 2: NIH/3T3 untreated
Fig2: ICC staining Phospho-Akt1(Ser473) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Phospho-Akt1(Ser473) antibody. Counter stained with hematoxylin.
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