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Fig1: Western blot analysis of Desmin on different lysates using anti-Desmin antibody at 1/1,000 dilution. Positive control:
Lane 1: Human skeletal muscle
Lane 2: Mouse heart
Lane 1: Human skeletal muscle
Lane 2: Mouse heart
Fig2: ICC staining Desmin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Desmin antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Desmin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Desmin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Desmin antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of C2C12 cells with Desmin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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