Fig1: Western blot analysis of NQO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate, untreated
Lane 2: A549 cell lysate, untreated

Fig2: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with EM1801-21 at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with EM1801-21 at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
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杭州華安生物技術(shù)有限公司成立于2007年3月,總部位于中國(guó)杭州,是一家從事抗體研發(fā)與生產(chǎn)的國(guó)家高新技術(shù)企業(yè)。公司致力于為全球的科研工作者和工業(yè)客戶提供高品質(zhì)的抗體試劑及抗體服務(wù)。
公司的產(chǎn)品線包括兔單抗、小鼠單抗、兔多抗、二抗、蛋白/多肽、基因敲除細(xì)胞系、抗體檢測(cè)用相關(guān)試劑等。2015年,公司開(kāi)始專注于高通量重組兔單克隆抗體的研發(fā)與產(chǎn)業(yè)化生產(chǎn)。
同時(shí),公司也為客戶提供定制抗體的開(kāi)發(fā)、純化和篩選服務(wù)。迄今為止,公司已累計(jì)完成超過(guò) 3000個(gè)定制抗體項(xiàng)目,涉及植物、動(dòng)物、醫(yī)學(xué)、水產(chǎn)、病毒等不同的科研領(lǐng)域。用戶使用公司抗體在Science、Immunity、Nature Communications、Autophagy、Plant Cell、Cell Research等雜志上發(fā)表文章。
公司不但專注于自身技術(shù)體系的完善和升級(jí),還注重推動(dòng)產(chǎn)學(xué)研合作:與浙江大學(xué)、四川大學(xué)、西南大學(xué)、中國(guó)科學(xué)院遺傳與發(fā)育生物學(xué)研究所等知名院校共同承擔(dān)省級(jí)和國(guó)家級(jí)課題,與杭州精準(zhǔn)醫(yī)藥研究中心進(jìn)行深度合作,進(jìn)行技術(shù)成果交流和共享。
Hangzhou HuaAn Biotechnology Co., Ltd (HuaBio) was founded in 2007. Our headquarter is located in Hangzhou, China. With over 3,000 antibodies developed, HuaBio is committed to providing high-quality antibodies and antibody services to our customers. We have products including recombinant rabbit monoclonal antibodies, mouse monoclonal antibodies, rabbit polyclonal antibodies, phosphor-specific antibodies, secondary antibodies.
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