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Fig1: Western blot analysis of CRK on human CRK recombinant protein using anti-CRK antibody at 1/1,000 dilution.
Fig2: Western blot analysis of CRK on HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate using anti-CRK antibody at 1/1,000 dilution.
Fig3: ICC staining CRK (green) and actin filaments (red) in 3T3-L1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CRK antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CRK antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of MCF-7 cells with CRK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
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