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Fig1: Western blot analysis of MSI2 on different lysates using anti-MSI2 antibody at 1/1,000 dilution. Positive control:
Lane 1: Lovo
Lane 2: PC-12
Lane 1: Lovo
Lane 2: PC-12
Fig2: ICC staining MSI2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining MSI2 in Lovo cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of Hela cells with MSI2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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