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Fig1: Western blot analysis of IRAKM on human IRAKM recombinant protein using anti- IRAKM antibody at 1/1,000 dilution.
Fig2: Western blot analysis of IRAKM on HEK293 (1) and IRAKM -hIgGFc transfected HEK293 (2) cell lysate using anti-IRAKM antibody at 1/1,000 dilution.
Fig3: ICC staining IRAKM (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining IRAKM (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- IRAKM antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- IRAKM antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of HepG2 cells with IRAKM antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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