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Fig1: Western blot analysis of WNT3A on human WNT3A recombinant protein using anti-WNT3A antibody at 1/1,000 dilution.
Fig2: Western blot analysis of WNT3A on HEK293 (1) and WNT3A-hIgGFc transfected HEK293 (2) cell lysate using anti-WNT3A antibody at 1/1,000 dilution.
Fig3: Western blot analysis of WNT3A on different cells lysate using anti-WNT3A antibody at 1/1,000 dilution. Positive control： Line1: A549 Line2: HepG2 Line3: A431
Fig4: ICC staining WNT3A (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-WNT3A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-WNT3A antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with WNT3A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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