Fig1: Western blot analysis of Bid on human thymus cells lysates using anti-Bid antibody at 1/500 dilution.
Fig2: ICC staining Bid in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Bid in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Bid in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bid antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bid antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Bid antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bid antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Bid antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of Hela cells with Bid antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary
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