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Fig1: Western blot analysis of Phospho-POLR2A (S5) on different cells lysates using anti- Phospho-POLR2A (S5) antibody at 1/500 dilution. Positive control：
Line 1: Hela
Line 2: MCF-7
Line 1: Hela
Line 2: MCF-7
Fig2: ICC staining Phospho-POLR2A (S5) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Phospho-POLR2A (S5) in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Phospho-POLR2A (S5) in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Phospho-POLR2A (S5) antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti- Phospho-POLR2A (S5) antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Phospho-POLR2A (S5) antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti- Phospho-POLR2A (S5) antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of Hela cells with Phospho-POLR2A (S5) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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