Fig1: Western blot analysis of TTR on different cells lysates using anti-TTR antibody at 1/500 dilution. Positive control：
Line 1: Human brain
Line 2；Human lung
Line 1: Human brain
Line 2；Human lung
Fig2: ICC staining TTR in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining TTR in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TTR antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- TTR antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-TTR antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TTR antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TTR antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of MCF-7 cells with TTR antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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