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Fig1: Western blot analysis of p-HSF1(S326) on different lysates using anti-p-HSF1(S326) antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: BT20
Lane 3: AGS
Lane 1: Hela
Lane 2: BT20
Lane 3: AGS
Fig2: ICC staining p-HSF1(S326) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining p-HSF1(S326) in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining p-HSF1(S326) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p-HSF1(S326) antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with p-HSF1(S326) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary
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