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Fig1: Western blot analysis of E Cadherin on MCF-7 (1) and A431 (2) cells lysates using anti-E Cadherin antibody at 1/1,000 dilution.
Fig2: ICC staining E Cadherin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.
Fig3: ICC staining E Cadherin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.
Fig4: ICC staining E Cadherin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25 Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-E Cadherin antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of A431 cells with E Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
