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Fig1: Western blot analysis of Alpha-tubulin on different cell lysates using anti-Alpha -tubulin antibody at 1/10000 dilution. Positive control: Lane 1: NIH/3T3 Lane2: HepG2 Lane3: PC12 Lane 4: Hela
Fig2: ICC staining of Alpha-tubulin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining of Alpha-tubulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: IHC-P image of Alpha-tubulin staining with Alpha-tubulin antibody on tissue sections from human stomach. The secondary used was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Alpha-tubulin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Alpha-tubulin antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela?cells with Alpha-tubulin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
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