APE1 鼠單克隆抗體

英文名稱：	APE1	總訪問(wèn)：	121
國(guó)產(chǎn)/進(jìn)口：	國(guó)產(chǎn)	半年訪問(wèn)：	20
產(chǎn)地/品牌：	華安生物/HUABIO	產(chǎn)品類別：	抗體
規(guī) 格：	鼠單克隆抗體	最后更新：	2021-4-20
貨號(hào)：	EM1801-12
CAS 號(hào)：
參考報(bào)價(jià)：	2500/100ul
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銷售商：杭州華安生物技術(shù)有限公司

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Fig1: Western blot analysis of APE1 on HL-60 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Fig2: ICC staining APE1 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with APE1 monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Fig3: ICC staining APE1 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with APE1 monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APE1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with EM1801-12 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-APE1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with EM1801-12 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-APE1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with EM1801-12 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-APE1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with EM1801-12 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Fig8: Flow cytometric analysis of APE1 was done on SiHa cells. The cells were fixed, permeabilized and stained with APE1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.

杭州華安生物技術(shù)有限公司成立于2007年3月，總部位于中國(guó)杭州，是一家從事抗體研發(fā)與生產(chǎn)的國(guó)家高新技術(shù)企業(yè)。公司致力于為全球的科研工作者和工業(yè)客戶提供高品質(zhì)的抗體試劑及抗體服務(wù)。
公司的產(chǎn)品線包括兔單抗、小鼠單抗、兔多抗、二抗、蛋白/多肽、基因敲除細(xì)胞系、抗體檢測(cè)用相關(guān)試劑等。2015年，公司開(kāi)始專注于高通量重組兔單克隆抗體的研發(fā)與產(chǎn)業(yè)化生產(chǎn)。
同時(shí)，公司也為客戶提供定制抗體的開(kāi)發(fā)、純化和篩選服務(wù)。迄今為止，公司已累計(jì)完成超過(guò) 3000個(gè)定制抗體項(xiàng)目，涉及植物、動(dòng)物、醫(yī)學(xué)、水產(chǎn)、病毒等不同的科研領(lǐng)域。用戶使用公司抗體在Science、Immunity、Nature Communications、Autophagy、Plant Cell、Cell Research等雜志上發(fā)表文章。
公司不但專注于自身技術(shù)體系的完善和升級(jí)，還注重推動(dòng)產(chǎn)學(xué)研合作：與浙江大學(xué)、四川大學(xué)、西南大學(xué)、中國(guó)科學(xué)院遺傳與發(fā)育生物學(xué)研究所等知名院校共同承擔(dān)省級(jí)和國(guó)家級(jí)課題，與杭州精準(zhǔn)醫(yī)藥研究中心進(jìn)行深度合作，進(jìn)行技術(shù)成果交流和共享。

Hangzhou HuaAn Biotechnology Co., Ltd (HuaBio) was founded in 2007. Our headquarter is located in Hangzhou, China. With over 3,000 antibodies developed, HuaBio is committed to providing high-quality antibodies and antibody services to our customers. We have products including recombinant rabbit monoclonal antibodies, mouse monoclonal antibodies, rabbit polyclonal antibodies, phosphor-specific antibodies, secondary antibodies.

HuaBio is also committed to providing the highest quality in customized antibody services . By now, we have delivered more than 3000 customized antibodies for specific applications in various fields (e.g.Neuroscience, Epigenetics, Stem Cells, Microbiology, Signal Transduction Apoptosis and etc. )

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