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Fig1: Western blot analysis of TRAFD1 on human TRAFD1 recombinant protein using anti-TRAFD1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of TRAFD1 on HEK293 (1) and TRAFD1-hIgGFc transfected HEK293 (2) cell lysate using anti-TRAFD1 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of TRAFD1 on different cell lysate using anti-TRAFD1 antibody at 1/1,000 dilution.
Positive control：
Line1:HEK293 Line2:Raji Line3:Jurkat
Positive control：
Line1:HEK293 Line2:Raji Line3:Jurkat
Fig4: ICC staining TRAFD1 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of Hela cells with TRAFD1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).
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