植物細(xì)胞膜H+-ATPase，廣泛分布于植物細(xì)胞質(zhì)膜上的ATP酶，由Hodges等人于1972年研究質(zhì)膜微囊的ATPase的水解活力時(shí)發(fā)現(xiàn)。根據(jù)功能和位置不同，植物細(xì)胞內(nèi)的H+-ATPase可分為F、P和V三大類型：F型ATPase主要分布在線粒體和葉綠體上；P型ATPase分布在質(zhì)膜上；V型ATPase分布在液泡膜、內(nèi)質(zhì)網(wǎng)膜、溶酶體上。今天的主角是，P型H+-ATPase！（下文提及均為P型H+-ATPase）

 

質(zhì)膜H+-ATP酶（P型H+-ATPase）是一個(gè)約100kDa的蛋白質(zhì)家族，被認(rèn)為是植物和真菌質(zhì)膜的專屬蛋白。該蛋白被固定在生物膜內(nèi)，形成電化學(xué)梯度，作為能量來(lái)源，對(duì)大多數(shù)代謝物的吸收和植物對(duì)環(huán)境的反應(yīng)（如葉子的移動(dòng)，氣孔開合，花粉管生長(zhǎng)..）至關(guān)重要。

 

 

因?yàn)镠+-ATPase極其重要，且廣泛分布在各類植物物種中，因此，絕大多數(shù)植物研究工作者的科學(xué)研究，幾乎都逃不開一支靠譜的，多應(yīng)用，多文章的H+-ATPase抗體。

 

H+-ATPase實(shí)驗(yàn)結(jié)果展示：

 

WB：1: 5000	IF：1: 300

 

使用H+-ATPase抗體，發(fā)高分文章：

 

Wang et al. (2020). Plant NLR Immune Receptor Tm-22 Activation Requires NB-ARC Domain-Mediated Self-Association of CC Domain. PLoS Pathog. 2020 Apr 27;16(4):e1008475. doi: 10.1371/journal.ppat.1008475.

 

Collins et al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172

 

Wang et al. (2020). The Arabidopsis exocyst subunits EXO70B1 and EXO70B2 regulate FLS2 homeostasis at the plasma membrane. New Phytol. 2020 Mar 2. doi: 10.1111/nph.16515.

 

Kuang et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.

 

Yuan et al. (2019). Phospholipidase Dδ Negatively Regulates the Function of Resistance to Pseudomonas syringae pv. Maculicola 1 (RPM1). Front Plant Sci. 2019 Jan 18;9:1991. doi: 10.3389/fpls.2018.01991.

 

Zhang et all. (2018). Root plasma membrane H+-ATPase is involved in low pH-inhibited nitrogen accumulation in tea plants (Camellia sinensis L.). Plant Growth Regul (2018) 86: 423.

 

Roth et al. (2018). A rice Serine/Threonine receptor-like kinase regulates arbuscular mycorrhizal symbiosis at the peri-arbuscular membrane. Nat Commun. 2018 Nov 8;9(1):4677. doi: 10.1038/s41467-018-06865-z.

 

Wang et al. (2018). Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018 Nov 16. pii: 201813058. doi: 10.1073/pnas.1813058115.

 

Pertl-Obermeyer et al. (2018). Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells. PLoS One. 2018 Aug 29;13(8):e0201480. doi: 10.1371/journal.pone.0201480.

 

Zhang et al. (2018). Maintenance of mesophyll potassium and regulation of plasma membrane H+-ATPase are associated with physiological responses of tea plants to drought and subsequent rehydration. The Crop Journal July 2018. (Camellia sinensis)

 

Seguel et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941

 

Duan et al. (2017). A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and Activates Glyoxalase I Expression during Drought Stress. Plant Cell. 2017 Jul;29(7):1748-1772. doi: 10.1105/tpc.17.00044. (Nicotiana benthamiana)

 

Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.

 

Aloui et al. (2017). The plasma membrane proteome of Medicago truncatula roots as modified by arbuscular mycorrhizal symbiosis. Mycorrhiza. 2017 Jul 19. doi: 10.1007/s00572-017-0789-5.

 

Lomin et al. (2017). Studies of cytokinin receptor–phosphotransmitter interaction provide evidences for the initiation of cytokinin signalling in the endoplasmic reticulum. Functional Plant Biology, CSIRO Publications. (Nicotiana benthamiana, western blot)

 

Kovaleva et al. (2017). Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets. DOI:10.17265/2161-6256/2016.04.004. (immunolocalization)

 

Liao et al. (2017). Arabidopsis E3 ubiquitin ligase PLANT U-BOX13 (PUB13) regulates chitin receptor LYSIN MOTIF RECEPTOR KINASE5 (LYK5) protein abundance. New Phytol. 2017 Feb 14. doi: 10.1111/nph.14472.

 

LaMontagne et al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020.

 

Heard et al. (2015). Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection. Mol Cell Proteomics. 2015 Jul;14(7):1796-813. doi: 10.1074/mcp.M115.050286. Epub 2015 Apr 21.

 

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