植物生長與各種常量和微量營養元素密切相關，所以測量各種常微量元素對植物的脅迫效應具有很重要的意義。大量的研究表明，Fv/Fm、Yield和ETR是與植物CO2固定能力密切相關的三個參數，所以在研究中倍受矚目。

但我們應該知道，植物營養元素的脅迫除非到了非常嚴重或者較為嚴重的程度才會影響到光系統II。這三個參數并不能反映所有的植物營養元素脅迫，有的可以反映輕度脅迫、有的只能反映中度脅迫、有的則只能反映非常嚴重或者較重程度的脅迫。

下面的內容指出了Fv/Fm和 Yield兩個參數在指示作用上的局限性。

 

 Fv/Fm 對非常低水平的氮素脅迫比較敏感。 (Baker 2004)

 Fv/Fm 對較為嚴重的硫素脅迫比較敏感。 (Baker 2004)

 Fv/Fm 對鎳元素脅迫不敏感。 (Joshi 2004)

 Fv/Fm 對鋅元素脅迫不敏感。(Joshi 2004)

 

 

Yield 對較為嚴重的硫素脅迫比較敏感。(Baker 2004)

注：Fv/Fm需要在暗適應條件下測量，可使用OPTIC公司的所有調制式熒光測量儀進行測量，包括OS-5P、OS1P 性價比很高的便攜式OS-30P；Yield的測量需要在光照條件下當葉片達到光合作用穩態的時候進行測量，可使用OPTIC公司的OS1-FL、OS5p進行測量。K-Step、 Performance Index、NPQ、qP、FRFex360/FRFex400等參數則只能使用OS5P進行測量。硫元素和氮元素脅迫可以通過葉綠素相對含量測定儀CCM200進行測量

 

 

 

 

 

 

 

 

Adams M.， Norvell A. Philpot D. Peverly J. (2000) Spectral detection of Micronutrient Deficiency in ‘Bragg’ Soybean， Agronomy J. 92:261-268

 

Cavender-Bares J. & Fakhri A. Bazzaz 2004 – “From Leaves to Ecosystem: Using Chlorophyll Fluorescence to Assess Photosynthesis and Plant Function in Ecological Studies”. Jeannine Cavender Bares， Fakhri A. Bazzaz， From Chapter 29，

 

“Chlorophyll a Fluorescence a Signature of Photosynthesis”， edited by George Papaqeorgiou and Govindjee， published by Springer 2004， PO Box 17， 3300 AA Dordrecht， The Netherlands， page 746-747 ETR Drought stress and npq

 

Baker N.R， Rosenquist E. (2004) Applications of chlorophyll fluorescence can improve crop production strategies: an examination of future possibilities， Journal of Experimental Botany， 55(403):1607-1621

 

Cheng L.， Fuchigami L.， Breen P.， (2001) “The relationship between photosystem II efficiency and quantum yield for CO2 assimilation is not affected by nitrogen content in apple leaves.” Journal of Experimental Botany， 52(362):1865-1872

 

Hermans C， Johnson GN， Strasser RJ， Verbruggen N (2004) Physiological characterization of magnesium deficiency in sugar beet: acclimation to low magnesium differentially affects photosystems I and II. Planta 220: 344-355.

 

Joshi M.K.， Mohanty P. (2004): Chlorophyll a fluorescence as a probe of heavy metal ion toxicity in plants. In: Papageorgiou G.C. (eds.): Chlorophyll Fluorescence: A Signature of Photosynthesis Advances in Photosynthesis and Respiration. Springer， Dordrecht: 637–661.

Kriedemann， P.E.， R.D. Graham， and J.T. Wiskich. (1985) Photosynthetic dysfunction and in vivo changes in chlorophyll a fluorescence from manganese-deficient wheat leaves. Aust. J. Agric. Res. 36: 157–169

 

Lanaras T. Moustakas M. Symeonidis L. Diamantoglou S.， & Karataglis S. (1993) Plant metal content， growth responses and some photosynthetic measurements on field – cultivated wheat growing on ore bodies enriched in Cu. Physiol Plant 88: 307-314

 

Samson G， Tremblay N.， Dudelzak A.E.， Babichenko S.M.， Dextraze L.， Wollring J.， (2000) NUTRIENT STRESS OF CORN PLANTS: EARLY DETECTION  AND DISCRIMINATION USING A COMPACT MULTIWAVELENGTH  FLUORESCENT LIDAR Proceedings of EARSeL-SIG-Workshop LIDAR， Dresden/FRG，  June 16 – 17

 

Strasser R.J， Tsimilli-Michael M.， and Srivastava A. (2004) - Analysis of Chlorophyll a Fluorescence Transient. From Chapter 12， “Chlorophyll a Fluorescence a Signature of Photosynthesis”， edited by George Papaqeorgiou and Govindjee， published by Springer 2004， PO Box 17， 3300 AA Dordrecht， The Netherlands， page 340

 

Stark Z.， Niemyska B.， Bogdan J.， Tawalbeh R.N. (2000) Response of Tomato Plants to Chilling Stress in Association with Nutrient or Phosphorus Starvation // Plant Soil.. V. 226. P. 99–106.

 

Tripathy BC， Mohanty P.， (1980) Zinc-inhibited electron transport of photosynthesis in isolated barley chloroplasts. Plant Physiology 66:1174-1178 

 

Tripathy BC， Bhatia B.， Mohanty P.， (1981) Inactivation of chloroplast photosynthetic electron transport activity by Ni ++. Biochim Biophys Acta 638:217-224

 

Tripathy BC， Bhatia B.， Mohanty P.， (1983) Cobalt ions inhibit electron-transport activity in Photosystem II without affecting Photosystem I Biochim Biophys Acta 722: 88-93

 

Weng X-Y.， Zheng C-J.， Xu H-X， Sun J-Y.， (2008) Characteristics of photosynthesis and functions of the water-water cycle in rice (Oryza sativa) leaves in response to potassium deficiency， Physiologia Plantarum Vol 131 Issue 4 PP 614-621